Comparison of Resin Infiltration Technique with Conventional Preventive Applications on Occlusal Fissures: EDS and SEM Analyses

Autor: Sidika Aynur Horuztepe, Esra Ergin, Alev Onen, Sevil Gürgan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Acta stomatologica Croatica : International journal of oral sciences and dental medicine
Volume 54
Issue 4
ISSN: 1846-0410
0001-7019
Popis: Svrha rada: Usporedba gubitka minerala ispod površine koji sprječava svojstvo infiltracije smole s topikalnom primjenom fluora, te smole za pečaćenje fisura na demineralizirane okluzalne fisure u simuliranim oralnim uvjetima. Materijali i metode: Demineralizirane su okluzalne plohe 64 izvađena intaktna ljudska treća kutnjaka. Zatim su zubi klasificirani u četiri skupine prema preventivnoj tehnici (n =16): G1 – uzorci koji su upotrijebljeni kao kontrolna skupina bez preventivnog liječenja; G2 – lokalna primjena fluorida (APF Gel/DEEPAK); G3 – primjena smole za pečaćenje fisura (ClinproTMSealant/3MESPE); G4 – tehnika infiltracije smolom (Icon/DMG). Za osam uzoraka iz svake skupine analiziran je kemijski sastavi prije pH cikliranja. Preostalih osam zuba iz svake skupine podvrgnuto je cikliranju pH tijekom 15 dana kako bi se simulirali intraoralni uvjeti. Nakon toga uzorci su lomljeni nakon potapanja u tekući dušik te su rendgenskom spektrometrijom s energijskom disperzijom (EDS) izmjerene razine fluora (F), kalcija (Ca), fosfora (P) i omjer Ca/P-a ispod površine svakog uzorka. Podatci su statistički analizirani (p = 0,05). Učinci preventivnih postupaka na površinsku topografiju uzoraka procijenjeni su s pomoću skenirajućeg elektronskog mikroskopa (SEM). Rezultati: Nije bilo značajnih razlika između skupina u razinama fluora, kalcija i fosfora te u omjerima Ca/P-a prije cikliranja pH i poslije toga postupka (p > 0,05). Prema opažanjima na SEM-u, sva tri preventivna postupka bila su učinkovita tijekom cikliranja pH. Zaključak: Svojstvo sprječavanja gubitaka minerala ispod površine moglo se usporediti tehnikom infiltracije smole koja se primjenjuje na okluzalnim fisurama i pri topikalnoj primjeni fluora i smole za pečaćenje fisura. Tehnika infiltracije smole mogla bi biti valjana alternativa tradicionalnim preventivnim i restaurativnim mjerama za liječenje početnih karijesnih lezija na okluzalnim fisurama zato što ima dvije prednosti – bolju infiltraciju i retenciju smole.
Objective: To compare the subsurface mineral loss preventing capability of resin infiltration technique with topical fluoride and fissure sealant applications to demineralized occlusal fissures under simulated oral conditions. Materials and Methods: Occlusal surfaces of 64 extracted intact human third molars were demineralized. Next, the teeth were classified into four groups according to preventive applications (n = 16): G1, Specimens used as the control group with no preventive treatment; G2, Topical fluoride application (APF Gel/ DEEPAK); G3, Fissure sealant application (ClinproT-MSealant/ 3M ESPE); and G4, Resin infiltration technique (Icon/ DMG). Chemical compositions before pH cycling were evaluated for eight specimens from each group. The remaining eight teeth from each group were subjected to pH cycling for 15 days to simulate the oral conditions. Subsequently, the specimens were fractured after immersion in liquid nitrogen and the subsurface fluoride (F), calcium (Ca), phosphorus (P) levels, and Ca/P ratio of each specimen were measured using energy dispersive x-ray spectrometer (EDS). The data were subjected to statistical analysis (p = 0.05). The effects of preventive applications to surface topography of specimens were evaluated using scanning electron microscope (SEM). Results: There were no significant differences among the groups in subsurface F, Ca, and P levels and Ca/P ratios before or after pH cycling (p > 0.05). All three preventive applications were effective during pH cycling according to SEM observations. Conclusions: The subsurface mineral loss preventing capability of resin infiltration technique applied to occlusal fissures was comparable to topical fluoride and fissure sealant applications. Clinical significance: The resin infiltration technique could represent a valid alternative to traditionally used both preventive and restorative treatments for treating initial carious lesions on occlusal fissures, offering the advantages of better resin penetration and retention.
Databáze: OpenAIRE
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