A high performance liquid chromatography (HPLC) method with evaporative light scattering detector for quantififi cation of major phospholipids classes of donkey serum

Autor: Hisham I. Seri, Hassan Y. Naim, Tigani Hassan
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Veterinarski arhiv
Volume 80
Issue 3
ISSN: 1331-8055
0372-5480
Popis: An HPLC method with an evaporative laser scattering detector was used to quantify major phospholipids fractions in donkeys’ serum. Blood samples were collected bimonthly for a whole year from 20 donkeys (10 male and 10 female) from the Sudanese breed kept at the premises of the Central Veterinary Research Laboratory (CVRL), Soba. The method used made the excellent separation possible of phosphatidylglycerine (PG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and sphingomyelin (SM). in 27 minutes, including the regeneration of the column. The SM resulted in two peaks (saturated and unsaturated fatty acids), as described by other researchers. The method was unable to separate phosphatidylserine (PS), which appeared in one peak with phosphatidylinositol. There is a signififi cant difference in the level of PG, PE, PI and SM between females and males.The method fully discussed in this study and the obtained values of major phospholipids can be used for health control and diagnosis of diseases.
Visokotlačna tekućinska kromatografija s detektorom raspršenja svjetla u uparenom uzorku rabljena je za određivanje količine glavnih frakcija fosfolipida u magarećem serumu. Uzorci krvi bili su uzeti od 20 životinja (10 magaraca i 10 magarica sudanske pasmine) držanih u prostorima Središnjega veterinarskoga istraživačkoga laboratorija, Soba. Rabljena metoda omogućila je izvrsno odvajanje fosfatidilglicerola (FG), fosfatidiletanolamina (FE), fosfatidilinozitola (FI), fosfatidilkolina i sfifi ngomijelina (SM) za 27 minuta uključujući i regeneraciju kolone. Sfingomijelin je pokazivao dva vrška (zasićene i nezasićene masne kiseline) kao što je opisano od drugih autora. Rabljenom metodom nije se moglo odvojiti fosfatidilserin od fosfatidilinozitola. Ustanovljena je signifikantna razlika u razini FG, FE, FI i SM u mužjaka i ženki. U radu se iscrpno raspravlja o metodi, a dobivene vrijednosti glavnih fosfolipida mogu se upotrijebiti za kontrolu zdravlja i dijagnosticiranje bolesti.
Databáze: OpenAIRE
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