The effect of fasting on the concentrations of total proteins and of uric acid as well as on aminotransferase activity in duckling blood plasma

Autor: Ivana Tlak, Suzana Milinković-Tur, Zvonko Stojević, Jasna Piršljin
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Zdroj: Veterinarski arhiv
Volume 78
Issue 5
ISSN: 1331-8055
0372-5480
Popis: The effect of a six-day fasting period on the variations of total protein and uric acid concentrations as well as on the activities of aspartate aminotransferase [aspartate:2-oxoglutarate aminotransferase (AST); EC 2.6.1.1] and alanine aminotransferase [L-alanine:2-oxoglutarate aminotransferase (ALT); EC 2.6.1.2] was investigated in Peking Duck ducklings. At the age of 28 days, the ducklings were divided into two groups: a normally fed control group (n = 28), and a group that was submitted to a six-day long fast. Eight ducklings from the experimental group and seven ducklings belonging to the control group were sacrificed by decapitation after the 3rd, the 4th, the 5th, and the 6th day of fasting. The blood for analysis was sampled from the neck, simultaneously in the experimental and in the control group, heparin being used as the anticoagulant. In the blood plasma obtained, the concentrations of total proteins and of uric acid as well as the levels of AST and ALT activities were assessed by spectrophotometer. Over the entire trial period, the concentration of total proteins in the blood plasma of the fasting ducklings was significantly lower than the control values: on day 3, P = 0.0239, and on days 4, 5, and 6, P = 0.0012. After the period of five days of the trial, a significantly higher (P = 0.018) value of uric acid concentration was measured in the experimental ducklings than in the controls, but at the same time, the lowest uric acid concentration in the entire trial period was found in the control group. In relation to the control group, significantly lower AST activity was found after the 5th day of fasting (P = 0.0276), and lower ALT activity after the 4th day of fasting (P = 0.0410). The low concentrations of total proteins over the entire trial period as well as reduced activities of the afore mentioned enzymes were probably a consequence of a reduced protein regeneration in the body due to the deficiency of amino acids from the gastro-intestinal tract during fasting.
Istražen je utjecaj šestodnevnog gladovanja na koncentraciju ukupnih bjelančevina i mokraćne kiseline, te aktivnosti aspartat-aminotransferaze [aspartate:2-oxoglutarate aminotransferase (AST); EC 2.6.1.1] i alanin-aminotransferaze [L-alanine:2-oxoglutarate aminotransferase (ALT); EC 2.6.1.2] u krvnoj plazmi pačića pekinške patke. U dobi od 28 dana pačići su bili podijeljeni u dvije skupine: kontrolnu skupinu (n= 28) i pokusnu skupinu koja je podvrgnuta šestodnevnom gladovanju (n = 32). Nakon 3., 4., 5. i 6. dana gladovanja žrtvovano je po 8 pačića pokusne skupine i po 7 pačića kontrolne skupine. Krv za analizu uzimana je s heparinom kao antikoagulansom, istovremeno pokusnoj i kontrolnoj skupini. U dobivenoj krvnoj plazmi spektrofotometrijski su određene koncentracije ukupnih bjelančevina i mokraćne kiseline, te aktivnosti AST i ALT. Tijekom pokusnoga razdoblja koncentracija ukupnih bjelančevina u krvnoj plazmi izgladnjivanih pačića bila je značajno niža od kontrolnih vrijednosti i to 3. dana (P = 0,0239) te 4., 5. i 6. dana (P = 0,0012). Nakon petodnevnog pokusnog razdoblja u izgladnjivanih pačića utvrđena je značajno viša (P = 0,018) vrijednost koncentracije mokraćne kiseline nego u kontrolnoj skupini, no istodobno je u kontrolnoj skupini zabilježena najniža koncentracija mokraćne kiseline tijekom cijeloga pokusnoga razdoblja. U odnosu na kontrolnu skupinu značajno niža aktivnost AST zabilježena je nakon 5. dana gladovanja (P = 0,0276), a aktivnosti ALT nakon 4. dana gladovanja (P = 0,0410). Niska koncentracija ukupnih bjelančevina u pačića pokusne skupine i smanjena aktivnost navedenih enzima vjerojatno je posljedica smanjene obnove bjelančevina u organizmu zbog nedostatka aminokiselina iz probavnoga trakta u vrijeme gladovanja.
Databáze: OpenAIRE
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