Production of γ-Decalactone by Yeast Strains under Different Conditions
| Autor: | Dayana Pereira de Andrade, Beatriz Ferreira Carvalho, Rosane Freitas Schwan, Disney Ribeiro Dias |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Food Technology and Biotechnology Volume 55 Issue 2 |
| ISSN: | 1334-2606 1330-9862 |
| Popis: | γ-Decalactone is a flavour compound that when obtained by biotechnological production using microorganisms is classified as natural. The aim of this study is to evaluate various conditions for γ-decalactone production by tropical yeast strains Yarrowia lipolytica CCMA 0242 and Lindnera saturnus CCMA 0243. The growth of and γ-decalactone production by Y. lipolytica CCMA 0242 were higher in castor oil than in glycerol. γ-Decalactone production in single batch or fed-batch fermentation did not differ significantly. The γ-decalactone production by L. saturnus CCMA 0243 was better at initial pH=5, while the production by Y. lipolytica CCMA 0242 was better at initial pH=6. The yeast L. saturnus CCMA 0243 produced more γ-decalactone than Y. lipolytica CCMA 0242 under the same fermentation conditions. The crude glycerol was not an alternative substrate for γ-decalactone production by Y. lipolytica CCMA 0242. Castor oil at volume fraction of 30 % showed better results as a substrate. The strain L. saturnus CCMA 0243 showed better results of γ-decalactone production. This yeast species can be considered an alternative producer of γ-decalactone in biotechnological processes. γ-Dekalakton je sastojak arome koji se, ako je dobiven biotehnološkom proizvodnjom uz pomoć mikroorganizama, smatra prirodnim proizvodom. Svrha je ovoga rada bila ispitati različite uvjete proizvodnje γ-dekalaktona s pomoću tropskih sojeva kvasca Yarrowia lipolytica CCMA 0242 i Lindnera saturnus CCMA 0243. Rast kvasca Y. lipolytica CCMA 0242 i proizvodnja γ-dekalaktona bili su izraženiji u podlozi s ricinusovim uljem nego s glicerolom. Koncentracija γ-dekalaktona dobivena šaržnom fermentacijom nije se bitno razlikovala od one dobivene fermentacijom s pritokom supstrata. S pomoću kvasca L. saturnus CCMA 0243 proizvedeno je više γ-dekalaktona pri pH=5, a s pomoću soja Y. lipolytica CCMA 0242 pri pH=6. Soj L. saturnus CCMA 0243 proizveo je više γ-dekalaktona od soja Y. lipolytica CCMA 0242 pri istim uvjetima fermentacije. Sirovi glicerol nije dobar supstrat za proizvodnju γ-dekalaktona s pomoću soja Y. lipolytica CCMA 0242, jer su bolji rezultati postignuti u podlozi s 30 % ricinusovog ulja. Proizvodnja je γ-dekalaktona bila bolja s pomoću soja L. saturnus CCMA 0243, pa se taj kvasac može smatrati alternativnim proizvođačem γ-dekalaktona u biotehnološkim procesima. |
| Databáze: | OpenAIRE |
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