Cijeljenje tetivnih ozljeda uporabom G-90 na modelu kunića: biokemijska i histopatološka procjena

Autor: Amin Bigham-Sadegh, Mehran Arabi, Effat Karimi, Iraj Karimi, Ahmad Oryan
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Veterinarski arhiv
Volume 86
Issue 3
ISSN: 1331-8055
0372-5480
Popis: Tendon injuries are one of the most common and disabling acute orthopedic disorders. Several techniques are used to restore the mobility of patients, but all have significant limitations. In some studies utilization of biomaterials has been investigated in the healing of tendons, skin and nerves. The present study was designed to investigate the effects of G-90, as a stimulating factor agent, on the healing of the superficial digital flexor tendon (SDFT) of rabbits after 35 days post tenotomy and surgical repair. Twenty male rabbits, 12 months old and weighing 2.0 ± 0.5 kg were used in this study. All rabbits were anesthetized, and the superficial flexor tendons of both hind limbs were cut transversely and then sutured with a Bunnel-Mayer suture pattern. After suturing tendons and skin, 0.5 mL of normal saline was injected in the injured tendon area of the left leg and 0.5 mL earthworm extract (EW) G-90 was injected at a concentration of 10 mg/mL into the injured tendon area of the right leg. Every two weeks 4 rabbits were euthanized, and samples were collected and sent for histopathological and biomechanical evaluation. In the histopathological evaluation less inflammation, more maturity of fibrocytes and more aggregation of collagen fibers were observed in tendons treated with G-90 in comparison with untreated tendons. In the biomechanical evaluation the ultimate strength of tendons treated with G-90 was superior to untreated tendons. The findings of the present experiment clearly show that administration of G-90 could enhance the structural and biomechanical properties of the experimentally tenotomized SDFT in rabbits.
Ozljede tetiva predstavljaju jedan od najčešćih akutnih ortopedskih poremećaja što dovode do njihove funkcionalne oslabljenosti. Razvijeno je nekoliko postupaka za ponovnu uspostavu pokretnosti pacijenata, ali svi imaju znatna ograničenja. U nekim istraživanjima opisana je uporaba biomaterijala za cijeljenje ozljeda tetiva, kože i živaca. U ovom su radu istraženi učinci G-90, kao stimulacijskog čimbenika cijeljenja tetive površinskog digitalnog fleksora kunića 35 dana nakon tenotomije i kirurškog liječenja. U pokus je bilo uzeto 20 kunića u dobi od 12 mjeseci, tjelesne mase 2,0 ± 0,5 kg. Svim su kunićima pod anestezijom bile poprečno prerezane tetive površinskog digitalnog fleksora stražnjih nogu te potom spojene Bunnel-Mayer-ovim šavom. Nakon šivanja tetive i kože, 0,5 mL fiziološke otopine bilo je ubrizgano u područje tetivne ozljede lijeve noge, a 0,5 mL iscrpka G-90 kišne gujavice u koncentraciji od 10 mg/mL bilo je ubrizgano u područje tetivne ozljede desne noge. Svaka dva tjedna bila su eutanazirana 4 kunića te su im bili uzeti uzorci za patohistološke i biokemijske pretrage. Patohistološkom pretragom tetiva kunića kojima je bio primijenjen G-90 ustanovljena je slabija upala, veća zrelost fibrocita i veće nakupljanje kolagenih vlakana u usporedbi s tetivama kojima nije bio primijenjen G-90. Kod biokemijske procjene konačna čvrstoća tetiva obrađenih s G-90 bila je veća u odnosu na one neobrađene. Rezultati ovog pokusa jasno su pokazali da primjena G-90 može osnažiti strukturu i biokemijska svojstva tetiva kunića nakon tenotomije i kirurškog liječenja.
Databáze: OpenAIRE
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