Antibakterijsko djelovanje Halotana, Eukaliptola i narančina ulja
Autor: | Zoran Karlović, Ivica Anić, Ivana Miletić, Goranka Prpić-Mehičić, Sonja Pezelj-Ribarić, Teuta Maršan |
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Jazyk: | angličtina |
Rok vydání: | 2000 |
Předmět: | |
Zdroj: | Acta stomatologica Croatica : International journal of oral sciences and dental medicine Volume 34 Issue 3 |
ISSN: | 1846-0410 0001-7019 |
Popis: | Za pokus su rabljeni sojevi Staphylococcus aureus i Enterococcus faecalis. Bakterijski inokulum od 0,5 Mc Farlanda razrijeđen je fiziološkom otopinom u omjerima 1:10, 1:10 2, 1:10 3, 1:10 4, 1:10 5 i 1:10 6. Otapalo količine 0,2 ml pomiješano je s istom količinom bakterijske suspenzije. Za svaku bakterijsku vrstu učinjeno je 60 uzoraka tj. po 10 uzoraka za pojedino razrjeđenje. Uzorci su hermetički zatvoreni i ostavljeni 10 i 30 minuta. Količina od 0,1 ml pojedinog uzorka nasađena je na krvnu ploču i pohranjena u termostat 24 h na temperaturi od 37˚C. Postupak je ponovljen dva puta. Halotan je pokazao najveću antibakterijsku aktivnost. Uništio je sve koncentracije sojeva Staphylococcus aureus i Enterococcus faecalis. Eukaliptol je djelovao na sojeve Staphylococcus aureus, a narančino ulje nije pokazalo antibakterijski učinak na ispitivane sojeve. Staphylococcus aureus and Enterococcus faecalis species were used for the experiment. 0,5 McFarland bacterial inoculum was diluted using saline in proportions 1:10, 1:10 2, 1:10 3, 1:10 4, 1:10 5 and 1:10 6. 0.2 millilitre of solving agent was mixed with an equal quantity of bacterial suspension. Sixty specimens, 10 for each proportion, were prepared. Specimens were hermetically closed and left for 10 and 30 minutes. 0.1 millilitre of each specimen was planted to the blood agar and put in the thermostat at 37°C for 24 hours. The procedure was repeated twice. Halothane exhibited greatest antibacterial activity, destroying all concentrations of Staphylococcus aureus and Enterococcus faecalis. Eucalyptol showed activity towards Staphylococcus aureus, while orange oil did not show any antibacterial effect on the examined bacteria. |
Databáze: | OpenAIRE |
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