Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
| Autor: | Karol Alfaro, Paulina Bustos, Ciara O´Sullivan, Pablo Conejeros |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Food Technology and Biotechnology Volume 53 Issue 3 |
| ISSN: | 1334-2606 1330-9862 |
| Popis: | A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 μg/mL of STX, with a detection limit of 7.5 ng/mL. Razvijena je jednostavna metoda detekcije saksitoksina, jednog od glavnih sastojaka paralitičkog toksina školjkaša prisutnih u moru preplavljenom dinoflagelatima (tzv. crvena plima). Upotrebom najnovije generacije fluorescentne boje za dvolančanu DNA bilo je moguće razlikovati fluorescenciju aptamera specifičnog za saksitoksin pri različitim koncentracijama, zbog čega smo pretpostavili da se struktura aptamera promijenila nakon vezanja na saksitoksin. Razvijena metoda je jako brza, za određivanje je potreban mali volumen uzorka, te se prisutnost saksitoksina može odrediti u rasponu koncentracija od 15 ng/mL do 3 µg/mL, s donjom granicom detekcije od 7,5 ng/mL. |
| Databáze: | OpenAIRE |
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