| Autor: |
Franche, Claudine, Diouf, D., Laplaze, L., Auguy, F., Frutz, T., Rio, M., Duhoux, Emile, Bogusz, Didier |
| Jazyk: |
angličtina |
| Rok vydání: |
1998 |
| Předmět: |
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| Popis: |
The purpose of this study was to compare the control of expression of legume and nonlegume hemoglobin genes. We used the #Casuarina glauca$ and #Allocasuarina verticillata$ transformation system to examine the properties of the soybean (Ibc3), #Parasponia andersonii$, and #Trema tomentosa$ hemoglobin gene promoters in actinorhizal plants. Expression of the hemoglobin promoters gus genes was examined by fluorometric and histochemical assays. The fluorometric assays in various organs showed that the soybean and #P. andersonii$ promoters were most active in nodules whereas the #T. tomentosa$ promoter gave a very high activity in roots. The histochemical study showed that GUS activity directed by the soybean and the #P. andersonii$ gus chimeric genes appeared mainly confined to the infected cells of the #C. glauca$ and #A. verticillata$ nodules. The #T. tomentosa$ hemoglobin promoter was primarily expressed in the root's cortex and vascular tissue. The results indicate that the soybean, #P. andersonii$, and #T. tomentosa$ hemoglobin promoters retain their cell-specific expression in transgenic members of the #Casuarinaceae$, suggesting a close relationship between legume, #Ulmaceae$ member, and actinorhizal hemoglobin genes. The conservation of the mechanism for nodule-specific expression of soybean, #P. andersonii$, and #C. glauca$ and #A. verticillata$ hemoglobin genes is discussed in view of recent molecular phylogenetic data that suggest a single origin for the pre-disposition to form root nodule symbioses. (Résumé d'auteur) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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