| Autor: |
Perraut, R., Richard, V., Varela, M. L., Trape, Jean-François, Guillotte, M., Tall, A., Toure, A., Sokhna, Cheikh, Vigan-Womas, I., Mercereau-Puijalon, O. |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Popis: |
Background: Numerous Plasmodium falciparum antigens elicit humoral responses in humans living in endemic areas. Use of multiplex assays is a convenient approach to monitor the antibody response against multiple antigens, but to integrate multiplex assay-derived data with datasets, generated previously using ELISA, comparative studies are needed. This work compares antibody responses to two P. falciparum antigens monitored using both technologies. Methods: The IgG response against the merozoite surface protein-1 PfMSP1p19 and the PF13-DBL1 alpha 1 domain of the P. falciparum Erythrocyte Membrane Protein1, expressed by the rosette-forming parasite 3D7/PF13 (PF13), was investigated using ELISA and a MAGPIX (R)-Luminex duplex assay. Archived plasma samples collected before the rainy season from 217 villagers living in Ndiop, a Senegalese meso-endemic setting, were studied. ROC analysis was used to define the optimal antibody measure readout. Association of antibody levels with protection against clinical malaria was analysed using Poisson regression in a retrospective study from active case detection records performed during the 5.5-month transmission season that followed blood sampling. Results: There was a strong positive correlation (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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