Cell Size and Morphological Properties of Yeast Saccharomyces Cerevisiae in Relation to Growth Temperature
| Autor: | Zakhartsev, M., Reuss, M. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
TEMPERATURE DEPENDENCE
BUDDING YEAST ANAEROBIOSIS GROWTH TEMPERATURE METABOLISM CELL DIVISION SURFACE-TO-VOLUME RATIO GLUCOSE BIOMASS GROWTH RATE CYTOLOGY CELL DISRUPTION CELL SIZE INTRACELLULAR MORPHOLOGY NONHUMAN TEMPERATURE SENSITIVITY ARTICLE ANAEROBIC BATCH GROWTH CELL CYCLE TEMPERATURE INTRACELLULAR GRANULARITY SACCHAROMYCES CEREVISIAE CELL VOLUME CELL BUDDING GROWTH DEVELOPMENT AND AGING SURFACE AREA ANAEROBIC GROWTH CELLULAR VOLUME GLUCOSE INTAKE MAINTENANCE CELL COUNT FUNGAL BIOMASS CELLULAR SIZE FUNGUS GROWTH YEAST CELL FLOW CYTOMETRY CELL SUSPENSION BIOREACTORS OPTICAL DENSITY BIOREACTOR |
| Zdroj: | FEMS Yeast Res. FEMS Yeast Research |
| Popis: | Cell volume is an important parameter for modelling cellular processes. Temperature-induced variability of cellular size, volume, intracellular granularity, a fraction of budding cells of yeast Saccharomyces cerevisiae CEN.PK 113–7D (in anaerobic glucose unlimited batch cultures) were measured by flow cytometry and matched with the performance of the biomass growth (maximal specific growth rate (μmax), specific rate of glucose consumption, the rate of maintenance, biomass yield on glucose). The critical diameter of single cells was 7.94 μm and it is invariant at growth temperatures above 18.5◦C. Below 18.5◦C, it exponentially increases up to 10.2 μm. The size of the bud linearly depends on μmax, and it is between 50% at 5◦C and 90% at 31◦C of the averaged single cell. The intracellular granularity (side scatter channel (SSC)-index) negatively depends on μmax. There are two temperature regions (5–31◦C vs. 33–40◦C) where the relationship between SSC-index and various cellular parameters differ significantly. In supraoptimal temperature range (33–40◦C), cells are less granulated perhaps due to a higher rate of the maintenance. There is temperature dependent passage through the checkpoints in the cell cycle which influences the μmax. The results point to the existence of two different morphological states of yeasts in these different temperature regions. © FEMS 2018. All rights reserved. The experimental part of the research has been carried out in Institute of Biochemical Engineering (IBVT, University of Stuttgart, Germany) and has been funded by the transnational research initiative ‘Systems Biology of Microorganisms (SysMO)’ within network MOSES: ‘MicroOrganism Systems Biology: Energy and Saccharomyces cerevisiae’ [http://www.sysmo.net]. Additionally, the author would like to thank Prof.Peter Scheurich (Institute of Cell Biology and Immunology, University of Stuttgart, Germany) for the experimental support, Achim Hauck (IBVT, University of Stuttgart, Germany) and Dr.Xuelian Yang (Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing, China) for the research assistance, Dr.Pavlo Holenya (Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Germany) for the discussion of the results. |
| Databáze: | OpenAIRE |
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