| Autor: |
Abt, Dietmar J., Einsle, Oliver, Niessen, Holger, Krieger, Robert, Messerschmidt, Albrecht, Schink, Bernhard, Kroneck, Peter M. H. |
| Jazyk: |
angličtina |
| Rok vydání: |
2002 |
| Předmět: |
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| Popis: |
Crystals of the molybdo-/iron±sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N2/H2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water.Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 . |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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