| Popis: |
1. We used fluorescence imaging with the visible wavelength indicator fluo-3 to investigate the calcium responses to cholinergic ligands of honeybee Kenyon cells in primary culture.2. Application of acetylcholine ( ACh) or nicotine, but not pilocarpine, promoted a calcium influx into the cell body and neurites. The increase in intracellular calcium after ACh stimulation was blocked by tu-bungarotoxin. These results support previous histochemical studies that suggested the expression of nicotinic cholinergic receptors on Kenyon cells.3. After depolarization with high K+ solution fluorescence increasedin the somata and neurites, which indicates the presence ofvoltage-gated Ca *+ channels in Kenyon cell membranes. |
