| Popis: |
El objetivo del presente estudio fue determinar la presencia de agentes infecciosos mediante estudios microbiológicos e histopatológicos en tetra neón (Paracheirodon innesi), pez amazónico ornamental. Se colectaron 168 peces, al azar, del acuario de un acopiador minorista de peces ornamentales de Iquitos, Perú. En la necropsia de 84 peces se tomaron muestras de riñón y bazo para el aislamiento de bacterias en agar tripticasa de soya (TSA), Citophaga (AO) y Middlebrook 7H9, haciendo la identificación mediante coloración Gram y análisis bioquímico. El estudio histopatológico se hizo con los 84 peces restantes. Se aislaron bacterias de los géneros Flavobacterium, Staphylococcus, Carnobacterium, Pseudomonas, Enterococcus, Bacillus y de la familia Enterobacteriaceae. En branquias se observó hiperplasia lamelar, fusión de lamelas, así como presencia de esporas de Mixosporidia en los ápices de las lamelas; en intestino se encontró hiperplasia del epitelio y presencia de esporas de Microsporidium en enterocitos; en hígado se encontró degeneración hidrópica, grasa y presencia de un granuloma parasitario; en riñones se encontró edema peritubular y degeneración hidrópica tubular; y en músculo esquelético se encontró necrosis, granulomas y presencia de quistes de Mixosporidium y esporas de Microsporidium, Pleistophora y Heterosporis The aim of this study was to determine the presence of infectious agents by microbiological and histopathological studies in the neon tetra (Paracheirodon innesi), an Amazonian ornamental fish. A total of 168 fish were randomly collected from the aquarium of an ornamental fish retail collector in Iquitos, Peru. For the microbiological study, kidney and spleen samples were collected during the necropsy of 84 specimens, and bacteria were isolated in trypticase soy agar (TSA), Citophaga (AO) and Middlebrook 7H9, while identification was done through Gram staining and biochemical analysis. The histopathological study was conducted in the remaining 84 fish. Bacteria isolated were of gen er a Flavobacterium, Staphylococcus, Carnobacterium, Pseudomonas, Enterococcus, Bacillus and of the Enterobacteriaceae family. In gills was observed laminar hyperplasia, fusion of lamellae and Mixosporidia spores at the tips of the lamellae; in the intestine was observed epithelial hyperplasia of the bowel and spores of Microsporidium in the intestinal villi; in the liver was found hydropic and fatty degeneration and a parasitic granuloma; in kidneys was found peritubular edema and hydropic degeneration; and in the muscular skeletal was found necrosis, presence of Mixosporidium cysts, granulomas, and spores of Microsporidium, Pleistophora and Heterosporis |
