Detección de Synergistes jonesii y variantes genéticas en rumiantes de diferentes regiones geográficas

Autor: MCSWEENEY, CHRIS S., PADMANABHA, JAGADISH, HALLIDAY, MICHAEL J., HUBBARD, BEN, DIERENS, LEANNE, DENMAN, STUART E., SHELTON, H. MAX
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Tropical Grasslands-Forrajes Tropicales, Volume: 7, Issue: 2, Pages: 154-163, Published: AUG 2019
Popis: Leucaena leucocephala is a nutritionally rich forage tree legume that contains a toxic non-protein amino acid, mimosine, from which other toxic compounds 3,4-dihydroxypyridone (3,4-DHP) and 2,3-DHP are formed in the rumen. The rumen bacterium Synergistes jonesii is able to degrade these DHP isomers into non-toxic end products. In this study we developed new PCR-based assays to improve the specificity and sensitivity of detection of S. jonesii in the rumen. Using these new assays in a survey of ruminants from different countries, S. jonesii appeared to be ubiquitous rather than isolated geographically. The bacterium was present as a minor population (
Databáze: OpenAIRE