| Autor: |
Ili, Carmen G, Brebi, Priscilla, Garcia, Patricia, Leal, Pamela, Lopez, Jaime, Tapia, Oscar, Letelier, Pablo, Weber, Helga, Castillo, Jonathan, Roa, Juan C |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
International Journal of Morphology, Volume: 33, Issue: 2, Pages: 638-646, Published: JUN 2015 |
| Popis: |
Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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