Autor: |
Martínez T, Alejandra, González C, Carlos, Kawaguchi P, Fernando, Montoya, Rolando, Corvalán, Alejandro, Madariaga B, Jaime, Roa S, Jorge, García C, Apolinaria, Salgado, Fernando, Solar, Henry, Palma, Mariana |
Jazyk: |
Spanish; Castilian |
Rok vydání: |
2001 |
Předmět: |
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Zdroj: |
Revista médica de Chile, Volume: 129, Issue: 10, Pages: 1147-1153, Published: OCT 2001 |
Popis: |
Background: The genes cagA and vacA encode H pylori virulence factors. Aim: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. Material and methods: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA gerotyping was done using polymerase chain reactions. Results: The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1. Conclusions: In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2 (Rev Méd Chile 2001; 129: 1147-53 |
Databáze: |
OpenAIRE |
Externí odkaz: |
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