| Autor: |
Schellenberger, M.T., Bock, U., Hennen, J., Gröber-Becker, F., Walles, Heike, Blömeke, B. |
| Přispěvatelé: |
Publica |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Popis: |
Dendritic cell activation is a key event of the adverse outcome pathway (AOP) for skin sensitization. In an attempt to integrate the impact of the skin environment created by stratified keratinocyte layers on the activation of dendritic cells, we investigated whether available 3D reconstructed human epidermis (RHE) models can be cocultured together with THP-1 cells, as surrogate dendritic cells, to assess chemical-induced dendritic cell activation in vitro. For this purpose, THP-1 cells were placed underneath the RHE, which was topically exposed to increasing concentrations of the model sensitizer eugenol (dissolved in aceton-olive oil, 4:1). Cell surface expression of the costimulatory molecule CD86 and the adhesion molecule CD54 on THP-1 cells were analyzed by flow cytometry. Preliminary results are promising and we conclude that this approach may allow the investigation of compounds with varying physicochemical properties and exposure at the air liquid interface. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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