| Autor: |
OLIVEIRA, JANICE V., ALMEIDA, GLEYBSON C., NOGUEIRA, MARIANE C.B.L., AGUIAR JÚNIOR, FRANCISCO C.A., MELO, AUDENES O., SILVA, THIAGO D.S., SANTOS, NOEMIA P.S., MAGALHÃES, NEREIDE S.S., MELO, SEBASTIÃO J., FALCÃO, EMERSON P.S. |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Anais da Academia Brasileira de Ciências, Volume: 95 Supplement 1, Article number: e20211078, Published: 10 JUL 2023 |
| Popis: |
The aim of the present study was to perform in vitro and in vivo assessments of the antineoplastic action of 4-amino-pyrimidine encapsulated in liposomes. Liposomes were prepared and characterized for particle size and drug encapsulation and submitted to long-term stability tests. Cytotoxicity assays were performed in HeLa cells. Antineoplastic activity was investigated using the experimental sarcoma 180 tumor in Swiss albino mice. Encapsulation efficiency was 82.93 ± 0.04% and no significant changes were found with respect to particle size or pH after centrifugation and mechanical agitation tests. The in vitro results at concentration of 20 μg/mL indicated a considerable reduction in cell viability after treatment with encapsulated pyrimidine (75.91%). The in vivo assays using the compounds in encapsulated and free forms and 5-fluorouracil achieved tumor inhibition rates of 66.47 ± 26.8%, 50.46 ± 16.24% and 14.47 ± 9.22%, respectively. Mitotic counts demonstrated a greater reduction in the number of mitoses in animals treated with liposomal pyrimidine (32.15%) compared to those treated with the pyrimidine free (87.69%) and 5-fluorouracil (71.39%). This study demonstrated that the development of liposome formulations containing 4-amino-pyrimidine is a promising alternative for overcoming limitations related to the toxicity of current cancer treatment, ensuring greater therapeutic efficacy. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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