Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin

Autor: Silva, D.G., Fagliari, J.J., Garcia, T.B.
Jazyk: portugalština
Rok vydání: 2008
Předmět:
Zdroj: Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Volume: 60, Issue: 3, Pages: 766-768, Published: JUN 2008
Popis: The aim of this study was to compare three different selective enrichment broths: Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. The bacteriological procedure involved pre-enrichment stages in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment, culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antiserum. The effects of enrichment temperatures using RV broth were also evaluated (37ºC and 42ºC). SC broth was significantly more efficient in the isolation of Salmonella Dublin (P
Databáze: OpenAIRE