| Autor: |
Silva-Neto, Habdias A., Duarte-Junior, Gerson F., Bazílio, Gabriela Silvério, Parente-Rocha, Juliana Alves, Colugnati, Diego B., Borges, Clayton L., Coltro, Wendell K. T. |
| Jazyk: |
portugalština |
| Rok vydání: |
2023 |
| Zdroj: |
Química Nova, Volume: 46, Issue: 5, Pages: 476-481, Published: 26 JUN 2023 |
| Popis: |
This report describes the fabrication of a low-cost magnetic separator holder combining 3D printing and compact neodymium blocks for allowing magnetic extraction and purification of RNA from samples collected by nasopharyngeal swab from patients infected by SARS-CoV-2. The device was designed to contain 24 entrances for plastic microtubes in an arrangement like a commercial device. The proof of concept of the proposed device was successfully demonstrated through the sample extraction and purification of swab samples collected from eight patients suspected of SARS-CoV-2 infection. The sample preparation protocol was performed using a commercial kit containing magnetic beads and different solutions. The performance of the printed device was compared to a commercial magnetic separator, usually employed in the golden standard techniques. The fabrication of the 3D printed magnetic separator was completed under optimized printing conditions within 6 h at cost of 4 USD per unit. The RNA extracted from samples using both devices was analyzed by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and the achieved results have indicated no statistical different at confidence level of 95%. Based on the achievements, the use of 3D printing and neodymium blocks have demonstrated an alternative route to be used in routing analysis associated to COVID-19 diagnosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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