| Popis: |
Introduction: In the recent years there have been some serious discussions about the human papillomavirus as a cofactor in etiology of nonmelanoma skin cancers. Cutaneous HPV types have been established and associated with precancerous lesions, in situ carcinomas and nonmelanoma skin cancers in numerous studies, but the actual importance of the virus in etiology of these lesions remains unknown. The aim of this study was to analyse the expression of various HPV types in precancerous lesions (AK), in situ carcinomas (MB) and nonmelanoma skin cancers (SCC and BCC) and to investigate differences in expression of various HPV types according to tumor localization (face/trunk) and patient’s geographic distribution (two regions with different insolation). Methods and Materials: 130 paraffin embedded tissues, histologically verified as AK, MB, SCC and BCC were analysed. After DNA isolation, skin HPV genotypes were detected using RHA kit Skin (beta) HPV (Diassay, The Netherlands) and »high risk« genotypes using HPV screening kit (AID, Germany). Conclusion: Significant difference between HPV DNA expression in precancerous lesions/in situ carcinomas and invasive skin carcinomas was not found. Higher expression of HPV DNA was detected in invasive skin carcinomas (SCC and BCC) excised from photo-exposed skin of patients living in a city with lower number of sunny days per year (city of Zagreb). Highrisk HPV types (HPV 16 and 18) were not detected in NMSC, AK and MB. These results suggest the role of HPV in NMSC carcinogenesis but further investigations need to be conducted. |
