| Popis: |
Associations between bovine leukoctye antigen alleles 'DRB3.2*16 ' and '*23' and mastitis in Canadian Holsteins were investigated. In order to facilitate the genotyping of a large population, a rapid PCR screening method, multi-primer target-PCR (MPT-PCR) was developed. Four primers were combined in one PCR reaction to detect the presence of alleles ' DRB3.2*16' and '*23' simultaneously. Cows (n = 3157), from 56 herds monitored for mastitis for 18 months, were then genotyped by MPT-PCR. No associations were found between the alleles and clinical mastitis, mastitis at calving or somatic cell score. However, 'DRB3.2*23' was significantly associated with an increased occurrence of subclinical mastitis caused by bacterial pathogens (p |
