| Autor: |
Wood, Carla M. |
| Přispěvatelé: |
Gibbins, Ann M.V., Walton, J. S. |
| Jazyk: |
angličtina |
| Rok vydání: |
1997 |
| Předmět: |
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| Popis: |
This thesis is an investigation into the expression of chorion gonadotrophin (CG)-like molecules in early bovine conceptuses. Past work from this laboratory reported low level production of luteinizing hormone (LH)-like mRNA by early bovine conceptuses. Based on homology between published LH and CG sequences in both primates and equids, it was decided to determine if the LH-like mRNA molecules expressed by bovine conceptuses are also CG-like. Reverse transcription-polymerase chain reaction (RT-PCR) was carried out on RNA from early bovine conceptuses using both $\beta$LH and degenerate CG primers. The fragment amplified with $\beta$LH primers was sequenced and identified to be homologous with the published bovine LH sequence. Fragments amplified with degenerate CG primers were cloned into a plasmid vector and sequenced; there was no convincing overall homology with published LH or CG gene sequences. The fragment amplified with the degenerate CG primers exhibited considerable sequence homology to a region of glia-derived nexin genes (human 84%, rat 82%), expression of which has not previously been reported for bovine conceptuses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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