Effect of self-assembled nanofibrous silk/polycaprolactone layer on the osteoconductivity and mechanical properties of biphasic calcium phosphate scaffolds
| Autor: | Roohani-Esfahani, SI, Lu, ZF, Li, JJ, Ellis-Behnke, R, Kaplan, DL, Zreiqat, H |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Calcium Phosphates
Ceramics Bone Regeneration Osteoblasts Compressive Strength Tissue Engineering Tissue Scaffolds Polyesters Biomedical Engineering Nanofibers Silk Gene Expression Biocompatible Materials Bone and Bones Elasticity Body Fluids Extracellular Matrix Apatites Materials Testing Humans Stress Mechanical Porosity Cells Cultured |
| Popis: | We here present the first successful report on combining nanostructured silk and poly(ε-caprolactone) (PCL) with a ceramic scaffold to produce a composite scaffold that is highly porous (porosity ∼85%, pore size ∼500 μm, ∼100% interconnectivity), strong and non-brittle with a surface that resembles extracellular matrix (ECM). The ECM-like surface was developed by self-assembly of nanofibrous structured silk (20-80 nm diameter, similar to native collagen found in ECM) over a thin PCL layer which is coated on biphasic calcium phosphate (BCP) scaffolds. The effects of different concentrations of silk solution on the mechanical and physical properties of the scaffolds were also comprehensively examined. Our results showed that using silk only (irrespective of concentration) for the modification of ceramic scaffolds could drastically reduce the compressive strength of the modified scaffolds in aqueous media, and the modification made a limited contribution to improving scaffold toughness. Using PCL/nanostructured silk the compressive strength and modulus of the modified scaffolds reached 0.42 MPa (compared with 0.07 MPa for BCP) and ∼25 MPa (compared with 5 MPa for BCP), respectively. The failure strain of the modified scaffold increased more than 6% compared with a BCP scaffold (failure strain of less than 1%), indicating a transformation from brittle to elastic behavior. The cytocompatibility of ECM-like composite scaffolds was investigated by studying the attachment, morphology, proliferation and bone-related gene expression of primary human bone-derived cells. Cells cultured on the developed scaffolds for 7 days had significant up-regulation of cell proliferation (∼1.6-fold higher, P |
| Databáze: | OpenAIRE |
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