| Autor: |
Guo, Z, Yang, CT, Maritz, MF, Wu, H, Wilson, P, Warkiani, ME, Chien, CC, Kempson, I, Aref, AR, Thierry, B |
| Rok vydání: |
2019 |
| Popis: |
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim The therapeutic ratio of radiotherapy is limited by acute or chronic side effects with often severe consequences to patients. The microvasculature is a central player involved in both tumor responses and healthy tissue/organ radiological injuries. However, current preclinical vascular models based on 2D culture offer only limited radiobiological insight due to their failure in recapitulating the 3D nature experienced by endothelial cells within the human microvasculature. To address this issue, the use of a 3D microvasculature-on-a-chip microfluidic technology is demonstrated in radiobiological studies. Within this vasculogenesis model a perfusable network that structurally mimics the human microvasculature is formed and the biological response to ionizing radiation including cellular apoptosis, vessel tight adherens junction breakage, DNA double strand break, and repair is systematically investigated. In comparison to cells grown in a 2D environment, human umbilical vein endothelial cells in the 3D microvasculature-on-a-chip displays significant differences in biological responses, especially at high X-ray dose. This data confirms the feasibility of using microvascular-on-a-chip models for radiobiological studies. Such vasculogenesis models have strong potential to yield more accurate prediction of healthy tissue responses to ionizing radiation as well as to guide the development of risk-reducing strategies to prevent radiation-induced acute and long-term side-effects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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