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The upper temperature tolerance limits of four freshwater fish species, silver perch Bidyanus bidyanus, eastern rainbowfish Melanotaenia duboulayi, western carp gudgeon Hypseleotris klunzingeri, and rainbow trout Oncorhynchus mykiss, were determined using the critical thermal maximum (CTMaximum) method. The CTMaximum tests were carried out with unexposed fish and fish exposed to sublethal concentrations of endosulfan, chlorpyrifos, and phenol to determine whether or not the CTMaximum was affected. The CTMaximum temperature of B. bidyanus decreased by 2.8, 3.8, and 0.3°C on exposure to endosulfan, chlorpyrifos, and phenol, respectively. Similarly, in M. duboulayi, the CTMaximum was decreased by 4.1, 2.5, and 0°C, while in H. klunzingeri it decreased by 3.1, 4.3, and 0.1°C, respectively, and in O. mykiss by 4.8, 5.9, and 0.7°C, respectively. Exposure to sublethal test concentrations of endosulfan and chlorpyrifos caused significant (p ≤ 0.0001) reductions in CTMaximum values for all fish species compared to that of unexposed fish. However, exposure to phenol did not cause any significant (p ≥ 0.05) change of CTMaximum temperatures. © 2007 SETAC. |