| Autor: |
Tjhi, WC, Lee, KK, Hung, T, Tsang, IWH, Ong, YS, Bard, F, Racine, V |
| Rok vydání: |
2011 |
| Předmět: |
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| Popis: |
Most phenotype-identification methods in cell-based screening assume prior knowledge about expected phenotypes or involve intricate parameter-setting. They are useful for analysis targeting known phenotype properties; but need exists to explore, with minimum presumptions, the potentially-interesting phenotypes derivable from data. We present a method for this exploration, using clustering to eliminate phenotype-labelling requirement and GUI visualisation to facilitate parameter-setting. The steps are: outlier-removal, cell clustering and interactive visualisation for phenotypes refinement. For drug-siRNA study, we introduce an auto-merging procedure to reduce phenotype redundancy. We validated the method on two Golgi apparatus screens and showcase its contribution for better understanding of screening-images. Copyright © 2011 Inderscience Enterprises Ltd. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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