| Autor: |
Bishop, DP, Blanes, L, Wilson, AB, Wilbanks, T, Killeen, K, Grimm, R, Wenzel, R, Major, D, Macka, M, Clarke, D, Schmid, R, Cole, N, Doble, PA |
| Rok vydání: |
2017 |
| Předmět: |
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| Popis: |
© 2017 Elsevier B.V. The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300 nL min−1. The potential of the instrument to eliminate common nanoLC – ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n = 7), and the detection limit was 14 ng mL−1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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