Thiol-gelatin-norbornene bioink for laser‐based high‐definition bioprinting

Autor: Dobos, Agnes, Van Hoorick, Jasper, Steiger, Wolfgang, Gruber, Peter, Markovic, Marica, Andriotis, Orestis G, Rohatschek, Andreas, Dubruel, Peter, Thurner, Philipp J, Van Vlierberghe, Sandra, Baudis, Stefan, Ovsianikov, Aleksandr
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: ADVANCED HEALTHCARE MATERIALS
ISSN: 2192-2640
2192-2659
Popis: Two-photon polymerization (2PP) is a lithography-based 3D printing method allowing the fabrication of 3D structures with sub-micrometer resolution. This work focuses on the characterization of gelatin-norbornene (Gel-NB) bioinks which enables the embedding of cells via 2PP. The high reactivity of the thiol-ene system allows 2PP processing of cell-containing materials at remarkably high scanning speeds (1000 mm s(-1)) placing this technology in the domain of bioprinting. Atomic force microscopy results demonstrate that the indentation moduli of the produced hydrogel constructs can be adjusted in the 0.2-0.7 kPa range by controlling the 2PP processing parameters. Using this approach gradient 3D constructs are produced and the morphology of the embedded cells is observed in the course of 3 weeks. Furthermore, it is possible to tune the enzymatic degradation of the crosslinked bioink by varying the applied laser power. The 3D printed Gel-NB hydrogel constructs show exceptional biocompatibility, supported cell adhesion, and migration. Furthermore, cells maintain their proliferation capacity demonstrated by Ki-67 immunostaining. Moreover, the results demonstrate that direct embedding of cells provides uniform distribution and high cell loading independently of the pore size of the scaffold. The investigated photosensitive bioink enables high-definition bioprinting of well-defined constructs for long-term cell culture studies.
Databáze: OpenAIRE
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