| Popis: |
Plant-parasitic nematodes (PPN) surveys have been conducted in both agricultural and natural habitats, including samples from sugarcane (Kilimanjaro region of Tanzania) [1], coffee (Jimma region of Ethiopia), finger millet (Eldoret region of Kenya), corn (eastern province of Rwanda), sand dunes (coastal beach of Gran Canaria) and other selected places in Belgium and Netherlands. This revealed an enormous diversity of PPN and allowed us to link more than 50 morphologically characterised PPN species with ribosomal and mitochondrial molecular barcodes (18S, D2-D3 region of 28S and ITS and cox1 sequences). Major results include discovery of four new PPN species, namely Heterodera dunensis from Gran Canaria [2]; Paratylenchus n. sp. from the Netherlands, Pratylenchus rwandae from Rwanda [3] and Rotylenchus wimbii from Kenya [4]; first reports of four Paratylenchus species in Belgium, one Paratylenchus species in Europe [5] and Cryphodera from Africa; synonymisation of Tylenchorhynchus agri with T. crassicaudata [1], Rotylenchus rhomboides with R. goodeyi and Cacopaurus with Paratylenchus; and corrective reassignment of multiple barcodes on public database that were previously unidentified or incorrectly classified [5]. Furthermore, our study also provides, for the first time, full length mitogenome and ribosomal DNA sequences of R. goodeyi, revealing the presence of two mitogenome variants within a single population. This new method made it possible to explore the use of full mitogenome sequences, originating from individual nematodes, in PPN diagnostics |
