Phthalate esters affect maturation and function of primate testis tissue ectopically grafted in mice
| Autor: | Rodriguez-Sosa, Jose R, Bondareva, Alla, Tang, Lin, Avelar, Gleide F, Coyle, Krysta M, Modelski, Mark, Alpaugh, Whitney, Conley, Alan, Wynne-Edwards, Katherine, França, Luiz R, Meyers, Stuart, Dobrinski, Ina |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Urologic Diseases Apoptosis SCID Medical and Health Sciences Mice Endocrinology & Metabolism Phthalates Pregnancy Diethylhexyl Phthalate Testis Animals Inhibins Spermatogenesis Non-human primates Transplantation Heterologous Sertoli Cells Agricultural and Veterinary Sciences Contraception/Reproduction Leydig Cells Cell Differentiation Environmental Exposure Seminiferous Tubules Biological Sciences Macaca mulatta Spermatogonia Dibutyl Phthalate Germ Cells Testis development Steroidogenesis Prenatal Exposure Delayed Effects Female |
| Zdroj: | Molecular and cellular endocrinology, vol 398, iss 1-2 |
| Popis: | Di-n-Butyl (DBP) and Di-(2-EthylHexyl) (DEHP) phthalates can leach from daily-use products resulting in environmental exposure. In male rodents, phthalate exposure results in reproductive effects. To evaluate effects on the immature primate testis, testis fragments from 6-month-old rhesus macaques were grafted subcutaneously to immune-deficient mice, which were exposed to 0, 10, or 500 mg/kg of DBP or DEHP for 14 weeks or 28 weeks (DBP only). DBP exposure reduced the expression of key steroidogenic genes, indicating that Leydig cell function was compromised. Exposure to 500 mg/kg impaired tubule formation and germ cell differentiation and reduced numbers of spermatogonia. Exposure to 10 mg/kg did not affect development, but reduced Sertoli cell number and resulted in increased expression of inhibin B. Exposure to DEHP for 14 week also affected steroidogenic genes expression. Therefore, long-term exposure to phthalate esters affected development and function of the primate testis in a time and dosage dependent manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|