| Autor: |
Barajas, JF, Zargar, A, Pang, B, Benites, VT, Gin, J, Baidoo, EEK, Petzold, CJ, Hillson, NJ, Keasling, JD |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Barajas, JF; Zargar, A; Pang, B; Benites, VT; Gin, J; Baidoo, EEK; et al.(2018). Biochemical Characterization of β-Amino Acid Incorporation in FluvirucinB2Biosynthesis. ChemBioChem. doi: 10.1002/cbic.201800169. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/71d5d5kn |
| DOI: |
10.1002/cbic.201800169. |
| Popis: |
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Naturally occurring lactams, such as the polyketide-derived macrolactams, provide a diverse class of natural products that could enhance existing chemically produced lactams. Although β-amino acid loading in the fluvirucinB2polyketide pathway was proposed by a previously identified putative biosynthetic gene cluster, biochemical characterization of the complete loading enzymes has not been described. Here we elucidate the complete biosynthetic pathway of the β-amino acid loading pathway in fluvirucinB2biosynthesis. We demonstrate the promiscuity of the loading pathway to utilize a range of amino acids and further illustrate the ability to introduce non-native acyl transferases to selectively transfer β-amino acids onto a polyketide synthase (PKS) loading platform. The results presented here provide a detailed biochemical description of β-amino acid selection and will further aid in future efforts to develop engineered lactam-producing PKS platforms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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