A comprehensive analysis of RHOA mutation positive and negative angioimmunoblastic T-cell lymphomas by targeted deep sequencing, expression profiling and single cell digital image analysis
| Autor: | Butzmann, Alexandra, Sridhar, Kaushik, Jangam, Diwash, Kumar, Jyoti, Sahoo, Malaya Kumar, Shahmarvand, Nahid, Warnke, Roger, Rangasamy, Elumalai, Pinsky, Benjamin Alan, Ohgami, Robert Shigeo |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Tumor Lymphoma High-Throughput Nucleotide Sequencing Hematology Middle Aged T-Cell Isocitrate Dehydrogenase angioimmunoblastic T-cell lymphoma ras homolog family member A Medicinal and Biomolecular Chemistry Rare Diseases Immunoblastic Lymphadenopathy Mutation Genetics Humans Female Oncology & Carcinogenesis rhoA GTP-Binding Protein peripheral T-cell lymphoma Biomarkers Aged Cancer |
| Zdroj: | International journal of molecular medicine, vol 46, iss 4 |
| Popis: | Angioimmunoblastic T‑cell lymphoma (AITL) is a uniquely aggressive mature T‑cell neoplasm. In recent years, recurrent genetic mutations in ras homolog family member A (RHOA), tet methylcytosine dioxygenase 2 (TET2), DNA methyltransferase 3 alpha (DNMT3A) and isocitrate dehydrogenase [NADP(+)] 2 (IDH2) have been identified as associated with AITL. However, a deep molecular study assessing both DNA mutations and RNA expression profile combined with digital image analysis is lacking. The present study aimed to evaluate the significance of molecular and morphologic features by high resolution digital image analysis in several cases of AITL. To do so, a total of 18 separate tissues from 10patients with AITL were collected and analyzed. The results identified recurrent mutations in RHOA, TET2, DNMT3A, and IDH2, and demonstrated increased DNA mutations in coding, promoter and CCCTC binding factor (CTCF) binding sites in RHOA mutated AITLs vs. RHOA non‑mutated cases, as well as increased overall survival in RHOA mutated patients. In addition, single cell computational digital image analysis morphologically characterized RHOA mutated AITL cells as distinct from cells from RHOA mutation negative patients. Computational analysis of single cell morphological parameters revealed that RHOA mutated cells have decreased eccentricity (more circular) compared with RHOA non‑mutated AITL cells. In conclusion, the results from the present study expand our understanding of AITL and demonstrate that there are specific cell biological and morphological manifestations of RHOA mutations in cases of AITL. |
| Databáze: | OpenAIRE |
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