| Autor: |
Beppu, L, Yang, T, Luk, M, Newbury, RO, Palmquist, J, Dohil, R, Kurten, RC, Broide, DH, Aceves, SS |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Journal of Pediatric Gastroenterology and Nutrition, vol 61, iss 2 |
| Popis: |
Objectives: Eosinophilic esophagitis (EoE) is a chronic, antigen-mediated disease in children and adults associated with substantial esophageal remodeling and fibrosis. The expression of the remodeling-associated matrix metalloproteinases (MMPs) has not been previously detailed in EoE. Methods: MMP-2 and-14 expression and cellular localization were assessed using real-time quantitative polymerase chain reaction and immunohistochemistry/immunofluorescence in EoE fibroblasts, active and inactive pediatric EoE biopsies, and nondiseased control biopsies. The effect of transforming growth factor (TGF)-b1 treatment on MMP-2 expression in cultured esophageal epithelial (HET1A) cells was analyzed. Results: MMP-2 and-14 mRNA were expressed in EoE fibroblasts and biopsies. Proliferating epithelial cells produced MMP-14 more abundantly in EoE than in controls (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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