Labeling of monodisperse polystyrene microspheres with tightly bound51Cr

Autor: Hinrichs, RJ, Kenoyer, JL, Phalen, RF, Timothy Crocker, TT
Jazyk: angličtina
Rok vydání: 1978
Předmět:
Zdroj: Hinrichs, RJ; Kenoyer, JL; Phalen, RF; & Timothy Crocker, TT. (1978). Labeling of monodisperse polystyrene microspheres with tightly bound51Cr. American Industrial Hygiene Association Journal, 39(7), 570-575. doi: 10.1080/0002889778507810. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2783f606
DOI: 10.1080/0002889778507810.
Popis: Monodisperse radioactive polystyrene latex microspheres have been prepared by attachment of chromium-51 to latex spheres. Labeling was by emulsion polymerization of chromium acetylacetonate dissolved in styrene monomer using commercially available microspheres. This was accomplished with two successive polymerization steps: radiation excitation and radical polymerization initiated with potassium persulfate. After attachment of the label the particle suspension was purified by repeated centrifugation wash cycles to remove labile radioactivity. Results indicate a radioactive binding yield of greater than 80% to the particles. The stability of the label was tested in in vivo and in vitro leaching studies. In these tests, the activity leaching rate was estimated to be less than 0.2% per day. © 1978, Taylor & Francis Group, LLC. All rights reserved.
Databáze: OpenAIRE