| Autor: |
Kang, S H, Fung, M A, Gandour-Edwards, R, Reilly, D, Dizon, T, Grahn, J, Isseroff, R R |
| Jazyk: |
angličtina |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Kang, S H; Fung, M A; Gandour-Edwards, R; Reilly, D; Dizon, T; Grahn, J; et al.(2004). Heat shock protein 27 is expressed in normal and malignant human melanocytes in vivo. Journal of Cutaneous Pathology, 31(10), 665-671. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/9gk8g768 |
| Popis: |
Background: Heat shock proteins (HSPs) are a family of highly conserved proteins found ubiquitously in mammalian cells, believed to be regulators of normal cell physiology and the cellular stress response. In addition, the small 27-kDa heat shock protein (HSP27) has previously been found to be a differentiation marker for keratinocytes and a prognostic marker associated with increased survival in certain cancerous tumors. Methods: Using immunohistochemistry on routinely processed paraffin sections, we examined skin biopsies from 15 invasive melanomas, 13 intradermal nevi, and two compound nevi immunostained with a mouse monoclonal antibody to HSP27. In addition, cultured melanocytes were heat stressed at 45degreesC for I h and then fixed and immunostained in order to localize HSP27 expression intracellularly. Results: We found cytoplasmic and strong perinuclear staining of HSP27 in melanocytes in normal skin, in melanomas, and in nevi. Nuclear reactivity was absent, In addition, in cultured non-malignant melanocytes, HSP27 expression relocated from the cytoplasm to the nucleus with heat stress. Conclusions: To our knowledge, this investigation is the first to demonstrate that HSP27 is expressed in melanocytes in normal skin, in nevi, and in non-malignant cultured melanocytes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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