The hypothalamic-pituitary-gonadal axis controls muscle stem cell senescence through autophagosome clearance
| Autor: | Kim, Ji-Hoon, Park, Inkuk, Shin, Hijai R, Rhee, Joonwoo, Seo, Ji-Yun, Jo, Young-Woo, Yoo, Kyusang, Hann, Sang-Hyeon, Kang, Jong-Seol, Park, Jieon, Kim, Ye Lynne, Moon, Ju-Yeon, Choi, Man Ho, Kong, Young-Yun |
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| Rok vydání: | 2021 |
| Předmět: |
Aging
Muscle stem cell Physiology Stem Cells Sex steroid hormones 1.1 Normal biological development and functioning Clinical Sciences Hypothalamus Autophagosomes Skeletal Human Movement and Sports Sciences Cellular senescence Myoblasts Mice Underpinning research Pituitary Gland Muscle regeneration Autophagy Genetics Muscle Animals Regeneration Gonads Cellular Senescence |
| Zdroj: | Journal of cachexia, sarcopenia and muscle, vol 12, iss 1 |
| Popis: | BackgroundWith organismal aging, the hypothalamic-pituitary-gonadal (HPG) activity gradually decreases, resulting in the systemic functional declines of the target tissues including skeletal muscles. Although the HPG axis plays an important role in health span, how the HPG axis systemically prevents functional aging is largely unknown.MethodsWe generated muscle stem cell (MuSC)-specific androgen receptor (Ar) and oestrogen receptor 2 (Esr2) double knockout (dKO) mice and pharmacologically inhibited (Antide) the HPG axis to mimic decreased serum levels of sex steroid hormones in aged mice. After short-term and long-term sex hormone signalling ablation, the MuSCs were functionally analysed, and their aging phenotypes were compared with those of geriatric mice (30-month-old). To investigate pathways associated with sex hormone signalling disruption, RNA sequencing and bioinformatic analyses were performed.ResultsDisrupting the HPG axis results in impaired muscle regeneration [wild-type (WT) vs. dKO, P |
| Databáze: | OpenAIRE |
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