A multimarker QPCR-based platform for the detection of circulating tumour cells in patients with early-stage breast cancer
Autor: | Molloy, TJ, Devriese, LA, Helgason, HH, Bosma, AJ, Hauptmann, M, Voest, EE, Schellens, JHM, van't Veer, LJ |
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Rok vydání: | 2011 |
Předmět: |
Adult
enrichment early-stage breast cancer Immunomagnetic Separation Oncology and Carcinogenesis Breast Neoplasms and over Middle Aged Prognosis Neoplastic Cells Polymerase Chain Reaction CTC quantitative PCR 80 and over Circulating Public Health and Health Services Humans Female Prospective Studies Oncology & Carcinogenesis circulating tumour cell skin and connective tissue diseases Neoplasm Staging Aged |
Zdroj: | British journal of cancer, vol 104, iss 12 |
Popis: | BackgroundThe detection of circulating tumour cells (CTCs) has been linked with poor prognosis in advanced breast cancer. Relatively few studies have been undertaken to study the clinical relevance of CTCs in early-stage breast cancer.MethodsIn a prospective study, we evaluated CTCs in the peripheral blood of 82 early-stage breast cancer patients. Control groups consisted of 16 advanced breast cancer patients and 45 healthy volunteers. The CTC detection was performed using ErbB2/EpCAM immunomagnetic tumour cell enrichment followed by multimarker quantitative PCR (QPCR). The CTC status and common clinicopathological factors were correlated to relapse-free, breast cancer-related and overall survival.ResultsCirculating tumour cells were detected in 16 of 82 (20%) patients with early-stage breast cancer and in 13 out of 16 (81%) with advanced breast cancer. The specificity was 100%. The median follow-up time was 51 months (range: 17-60). The CTC positivity in early-stage breast cancer patients resulted in significantly poorer relapse-free survival (log rank test: P=0.003) and was an independent predictor of relapse-free survival (multivariate hazard ratio=5.13, P=0.006, 95% CI: 1.62-16.31).ConclusionThe detection of CTCs in peripheral blood of early-stage breast cancer patients provided prognostic information for relapse-free survival. |
Databáze: | OpenAIRE |
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