| Autor: |
Kullmann, F, Judex, M, Ballhorn, W, Jüsten, HP, Wessinghage, D, Welsh, J, Yen, TJ, Lang, B, Hittle, JC, McClelland, M, Gay, S, Schölmerich, J, Müller-Ladner, U |
| Jazyk: |
angličtina |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Kullmann, F; Judex, M; Ballhorn, W; Jüsten, HP; Wessinghage, D; Welsh, J; et al.(1999). Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts. Arthritis Research, 1(1), 71-80. doi: 10.1186/ar13. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/5wp5g3hk |
| DOI: |
10.1186/ar13. |
| Popis: |
Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)-polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse transcription PCR, immunohistochemistry and in situ hybridization. CENP-E expression was independent from prednisolone and could not be completely inhibited by serum starvation. RAP-PCR is a suitable method to identify differentially expressed genes in rheumatoid synovial fibroblasts. Also, because motifs of CENP-E show homologies to jun and fos oncogene products and are involved in virus assembly, CENP-E may be involved in the pathophysiology of RA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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