Optimal protection against Salmonella infection requires noncirculating memory
| Autor: | Benoun, Joseph M, Peres, Newton G, Wang, Nancy, Pham, Oanh H, Rudisill, Victoria L, Fogassy, Zachary N, Whitney, Paul G, Fernandez-Ruiz, Daniel, Gebhardt, Thomas, Pham, Quynh-Mai, Puddington, Lynn, Bedoui, Sammy, Strugnell, Richard A, McSorley, Stephen J |
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| Rok vydání: | 2018 |
| Předmět: |
Salmonella typhimurium
and promotion of well-being T-Lymphocytes Cells tissue-resident memory Inbred C57BL Salmonella infection Vaccine Related Mice protective immunity Rare Diseases Biodefense Animals Cultured Liver Disease Prevention CD4 T cell Th1 Cells vaccines Foodborne Illness Prevention of disease and conditions Infectious Diseases Emerging Infectious Diseases Liver 3.4 Vaccines Salmonella Infections Immunization Female Digestive Diseases Infection Immunologic Memory Biotechnology |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 115, iss 41 |
| Popis: | While CD4 Th1 cells are required for resistance to intramacrophage infections, adoptive transfer of Th1 cells is insufficient to protect against Salmonella infection. Using an epitope-tagged vaccine strain of Salmonella, we found that effective protection correlated with expanded Salmonella-specific memory CD4 T cells in circulation and nonlymphoid tissues. However, naive mice that previously shared a blood supply with vaccinated partners lacked T cell memory with characteristics of tissue residence and did not acquire robust protective immunity. Using a YFP-IFN-γ reporter system, we identified Th1 cells in the liver of immunized mice that displayed markers of tissue residence, including P2X7, ARTC2, LFA-1, and CD101. Adoptive transfer of liver memory cells after ARTC2 blockade increased protection against highly virulent bacteria. Taken together, these data demonstrate that noncirculating memory Th1 cells are a vital component of immunity to Salmonella infection and should be the focus of vaccine strategies. |
| Databáze: | OpenAIRE |
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