Sister kinetochores are mechanically fused during meiosis I in yeast
| Autor: | Sarangapani, Krishna K, Duro, Eris, Deng, Yi, Alves, Flavia de Lima, Ye, Qiaozhen, Opoku, Kwaku N, Ceto, Steven, Rappsilber, Juri, Corbett, Kevin D, Biggins, Sue, Marston, Adèle L, Asbury, Charles L |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Microscopy
Saccharomyces cerevisiae Proteins Optical Tweezers Casein Kinase I General Science & Technology 1.1 Normal biological development and functioning Nuclear Proteins Cell Cycle Proteins Saccharomyces cerevisiae Chromatids Fluorescence Meiosis Underpinning research Genetics Generic health relevance Kinetochores |
| Zdroj: | Science (New York, N.Y.), vol 346, iss 6206 |
| Popis: | Production of healthy gametes requires a reductional meiosis I division in which replicated sister chromatids comigrate, rather than separate as in mitosis or meiosis II. Fusion of sister kinetochores during meiosis I may underlie sister chromatid comigration in diverse organisms, but direct evidence for such fusion has been lacking. We used laser trapping and quantitative fluorescence microscopy to study native kinetochore particles isolated from yeast. Meiosis I kinetochores formed stronger attachments and carried more microtubule-binding elements than kinetochores isolated from cells in mitosis or meiosis II. The meiosis I-specific monopolin complex was both necessary and sufficient to drive these modifications. Thus, kinetochore fusion directs sister chromatid comigration, a conserved feature of meiosis that is fundamental to Mendelian inheritance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|