| Autor: |
Song, Q, Jiao, K, Tonggu, L, Wang, LG, Zhang, SL, Yang, YD, Zhang, L, Bian, JH, Hao, DX, Wang, CY, Ma, YX, Arola, DD, Breschi, L, Chen, JH, Tay, FR, Niu, LN |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Science advances, vol 5, iss 3 |
| Popis: |
Contemporary models of intrafibrillar mineralization mechanisms are established using collagen fibrils as templates without considering the contribution from collagen-bound apatite nucleation inhibitors. However, collagen matrices destined for mineralization in vertebrates contain bound matrix proteins for intrafibrillar mineralization. Negatively charged, high-molecular weight polycarboxylic acid is cross-linked to reconstituted collagen to create a model for examining the contribution of collagen-ligand interaction to intrafibrillar mineralization. Cryogenic electron microscopy and molecular dynamics simulation show that, after cross-linking to collagen, the bound polyelectrolyte caches prenucleation cluster singlets into chain-like aggregates along the fibrillar surface to increase the pool of mineralization precursors available for intrafibrillar mineralization. Higher-quality mineralized scaffolds with better biomechanical properties are achieved compared with mineralization of unmodified scaffolds in polyelectrolyte-stabilized mineralization solution. Collagen-ligand interaction provides insights on the genesis of heterogeneously mineralized tissues and the potential causes of ectopic calcification in nonmineralized body tissues. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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