Dephosphorylation of the Ndc80 Tail Stabilizes Kinetochore-Microtubule Attachments via the Ska Complex
| Autor: | Cheerambathur, Dhanya K, Prevo, Bram, Hattersley, Neil, Lewellyn, Lindsay, Corbett, Kevin D, Oegema, Karen, Desai, Arshad |
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| Rok vydání: | 2017 |
| Předmět: |
cell division
chromosome segregation Microtubules Medical and Health Sciences Chromosomes Genetics Animals Spindle Poles Ska complex Phosphorylation Kinetochores Caenorhabditis elegans Caenorhabditis elegans Proteins Ndc80 complex mitosis Nonmammalian Biological Sciences GTP-Binding Protein alpha Subunits kinetochore cell polarity Embryo centromere Multiprotein Complexes Anaphase Microtubule-Associated Proteins Gene Deletion Protein Binding microtubule Developmental Biology |
| Zdroj: | Developmental cell, vol 41, iss 4 |
| Popis: | During cell division, genome inheritance is orchestrated by microtubule attachments formed at kinetochores of mitotic chromosomes. The primary microtubule coupler at the kinetochore, the Ndc80 complex, is regulated by Aurora kinase phosphorylation of its N-terminal tail. Dephosphorylation is proposed to stabilize kinetochore-microtubule attachments by strengthening electrostatic interactions of the tail with the microtubule lattice. Here, we show that removal of the Ndc80 tail, which compromises invitro microtubule binding, has no effect on kinetochore-microtubule attachments in the Caenorhabditis elegans embryo. Despite this, preventing Aurora phosphorylation of the tail results in prematurely stable attachments that restrain spindle elongation. This premature stabilization requires the conserved microtubule-binding Ska complex, which enriches at attachment sites prior to anaphase onset to dampen chromosome motion. We propose that Ndc80-tail dephosphorylation promotes stabilization of kinetochore-microtubule attachments via the Ska complex and that this mechanism ensures accurate segregation by constraining chromosome motion following biorientation on the spindle. |
| Databáze: | OpenAIRE |
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