Increased leucocyte apoptosis in transfused β-thalassaemia patients
| Autor: | Walter, Patrick B, Porter, John, Evans, Patricia, Kwiatkowski, Janet L, Neufeld, Ellis J, Coates, Thomas, Giardina, Patricia J, Grady, Robert W, Vichinsky, Elliott, Olivieri, Nancy, Trachtenberg, Felicia, Alberti, Daniele, Fung, Ellen, Ames, Bruce, Higa, Annie, Harmatz, Paul, Thalassemia Clinical Research Network |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Adolescent caspase beta-Thalassemia Immunology apoptosis DNA Fragmentation Cardiorespiratory Medicine and Haematology oxidant-stress Chelation Therapy Young Adult Clinical Research Bax inflammation Thalassemia Clinical Research Network Caspases Leukocytes Humans Blood Transfusion Female Child Preschool bcl-2-Associated X Protein |
| Zdroj: | British journal of haematology, vol 160, iss 3 |
| Popis: | This exploratory study assessed apoptosis in peripheral blood leucocytes (PBL) from β-thalassaemia patients receiving chronic transfusions and chelation therapy (deferasirox or deferoxamine) at baseline, 1, 6, and 12months. At baseline, thalassaemic PBLs presented 50% greater levels of Bax (BAX), 75% higher caspase-3/7, 48% higher caspase-8 and 88% higher caspase-9 activities and 428% more nucleosomal DNA fragmentation than control subjects. Only neutrophils correlated significantly with apoptotic markers. Previously, we showed that over the treatment year, hepatic iron declined; we now show that the ratio of Bax/Bcl-2 (BCL2), (-27·3%/year), and caspase-9 activity (-13·3%/year) declined in both treatment groups, suggesting that chelation decreases body iron and indicators of PBL apoptosis. |
| Databáze: | OpenAIRE |
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