Expression and targeting of transcription factor ATF5 in dog gliomas
| Autor: | York, D, Sproul, CD, Chikere, N, Dickinson, PJ, Angelastro, JM |
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| Rok vydání: | 2018 |
| Předmět: |
canine glioma cell lines
d/n-ATF5 Antibodies Cell Line Dogs Rare Diseases Genetics Animals Dog Diseases Veterinary Sciences astrocytoma brain tumour Cancer Tumor Brain Neoplasms Blotting Neurosciences Glioma oligodendroglioma Activating Transcription Factors Brain Disorders Brain Cancer 5.1 Pharmaceuticals Neoplasm Development of treatments and therapeutic interventions Western cell-penetrating peptide Biotechnology |
| Zdroj: | Veterinary and comparative oncology, vol 16, iss 1 |
| Popis: | BackgroundActivating transcription factor 5 (ATF5) is a transcription factor that is highly expressed in undifferentiated neural progenitor/stem cells as well as a variety of human cancers including gliomas.AimsIn this study, we examined the expression and localization of ATF5 protein in canine gliomas, and targeting of ATF5 function in canine glioma cell lines.Materials and methodsParaffin-embedded canine brain glioma tissue sections and western blots of tumours and glioma cells were immunoassayed with anti-ATF5 antibody. Viability of glioma cells was tested with a synthetic cell-penetrating ATF5 peptide (CP-d/n ATF5) ATF5 antagonist.ResultsATF5 protein expression was in the nucleus and cytoplasm and was present in normal adult brain and tumour samples, with significantly higher expression in tumours as shown by western immunoblotting. CP-d/n ATF5 was found to decrease cell viability in canine glioma cell lines in vitro in a dose-dependent manner.ConclusionSimilarities in expression of ATF5 in rodent, dog and human tumours, and cross species efficacy of the CP-d/n ATF5 peptide support the development of this ATF5-targeting approach as a novel and translational therapy in dog gliomas. |
| Databáze: | OpenAIRE |
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