| Autor: |
Mansoor, Saffar, Gupta, Navin, Luczy-Bachman, Georgia, Limb, G. Astrid, Kuppermann, Baruch D., Kenney, M. Cristina |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Mansoor, Saffar; Gupta, Navin; Luczy-Bachman, Georgia; Limb, G. Astrid; Kuppermann, Baruch D.; & Kenney, M. Cristina. (2013). Protective effects of lipoic acid on chrysene-induced toxicity on M?ller cells in vitro. Molecular Vision, 19, 25-38. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/8fn6h6vp |
| Popis: |
PurposeThis study evaluates the toxic effects of chrysene (a component from cigarette smoke) on Müller cells (MIO-M1) in vitro and investigates whether the inhibitor lipoic acid can reverse the chrysene-induced toxic effects.MethodsMIO-M1 cells were exposed to varying concentrations of chrysene with or without lipoic acid. Cell viability was measured by a trypan blue dye exclusion assay. Caspase-3/7 activity was measured by a fluorochrome assay. Lactate dehydrogenase (LDH) release was quantified by an LDH assay. The production of reactive oxygen/nitrogen species (ROS/RNS) was measured with a 2’,7’-dichlorodihydrofluorescein diacetate dye assay. Mitochondrial membrane potential (ΔΨm) was measured using the JC-1 assay. Intracellular ATP content was determined by the ATPLite kit.ResultsMIO-M1 cells showed significantly decreased cell viability, increased caspase-3/7 activity, LDH release at the highest chrysene concentration, elevated ROS/RNS levels, decreased ΔΨm value, and decreased intracellular ATP content after exposure to 300, 500, and 1,000 µM chrysene compared with the control. Pretreatment with 80 µM lipoic acid reversed loss of cell viability in 500-µM-chrysene-treated cultures (24.7%, p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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