Identification of Plasmodium dipeptidyl aminopeptidase allosteric inhibitors by high throughput screening
| Autor: | Sanchez, Mateo I, de Vries, Laura E, Lehmann, Christine, Lee, Jeong T, Ang, Kenny K, Wilson, Christopher, Chen, Steven, Arkin, Michelle R, Bogyo, Matthew, Deu, Edgar |
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| Přispěvatelé: | Carvalho, Luzia Helena |
| Rok vydání: | 2019 |
| Předmět: |
Cultured
General Science & Technology Cells Plasmodium falciparum Protozoan Proteins Cysteine Proteinase Inhibitors Preclinical Malaria Vector-Borne Diseases Antimalarials Rare Diseases Orphan Drug Infectious Diseases Good Health and Well Being Cysteine Proteases 5.1 Pharmaceuticals Humans Drug Evaluation Development of treatments and therapeutic interventions Dipeptidyl-Peptidases and Tripeptidyl-Peptidases Biotechnology |
| Zdroj: | PloS one, vol 14, iss 12 |
| Popis: | Dipeptidyl aminopeptidases (DPAPs) are cysteine proteases that cleave dipeptides from the N-terminus of protein substrates and have been shown to play important roles in many pathologies including parasitic diseases such as malaria, toxoplasmosis and Chagas's disease. Inhibitors of the mammalian homologue cathepsin C have been used in clinical trials as potential drugs to treat chronic inflammatory disorders, thus proving that these enzymes are druggable. In Plasmodium species, DPAPs play important functions at different stages of parasite development, thus making them potential antimalarial targets. Most DPAP inhibitors developed to date are peptide-based or peptidomimetic competitive inhibitors. Here, we used a high throughput screening approach to identify novel inhibitor scaffolds that block the activity of Plasmodium falciparum DPAP1. Most of the hits identified in this screen also inhibit Plasmodium falciparum DPAP3, cathepsin C, and to a lesser extent other malarial clan CA proteases, indicating that these might be general DPAP inhibitors. Interestingly, our mechanism of inhibition studies indicate that most hits are allosteric inhibitors, which opens a completely new strategy to inhibit these enzymes, study their biological function, and potentially develop new inhibitors as starting points for drug development. |
| Databáze: | OpenAIRE |
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