In vivo glucose imaging in multiple model organisms with an engineered single-wavelength sensor
| Autor: | Keller, Jacob P, Marvin, Jonathan S, Lacin, Haluk, Lemon, William C, Shea, Jamien, Kim, Soomin, Lee, Richard T, Koyama, Minoru, Keller, Philipp J, Looger, Loren L |
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| Rok vydání: | 2021 |
| Předmět: |
Central Nervous System
1.1 Normal biological development and functioning Medical Physiology Bioengineering transporters biosensor Imaging astrocyte Models Underpinning research Animals Humans glucose energy homeostasis Zebrafish Muscles Diabetes Neurosciences Proteins Biological Transport Biological neuron Rats HEK293 Cells Larva Three-Dimensional Neurological Drosophila Sprague-Dawley Biochemistry and Cell Biology Genetic Engineering Neuroglia metabolism |
| Zdroj: | Cell reports, vol 35, iss 12 |
| Popis: | Glucose is arguably the most important molecule in metabolism, and its dysregulation underlies diabetes. We describe a family of single-wavelength genetically encoded glucose sensors with a high signal-to-noise ratio, fast kinetics, and affinities varying over four orders of magnitude (1μM to 10mM). The sensors allow mechanistic characterization of glucose transporters expressed in cultured cells with high spatial and temporal resolution. Imaging of neuron/glia co-cultures revealed ∼3-fold faster glucose changes in astrocytes. In larval Drosophila central nervous system explants, intracellular neuronal glucose fluxes suggested a rostro-caudal transport pathway in the ventral nerve cord neuropil. In zebrafish, expected glucose-related physiological sequelae of insulin and epinephrine treatments were directly visualized. Additionally, spontaneous muscle twitches induced glucose uptake in muscle, and sensory and pharmacological perturbations produced large changes in the brain. These sensors will enable rapid, high-resolution imaging of glucose influx, efflux, and metabolism in behaving animals. |
| Databáze: | OpenAIRE |
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