| Autor: |
Chen, Wei-Wen, Lemieux, George A, Camp, Charles H, Chang, Ta-Chau, Ashrafi, Kaveh, Cicerone, Marcus T |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Nature chemical biology, vol 16, iss 10 |
| Popis: |
Caenorhabditis elegans serves as a model for understanding adiposity and its connections to aging. Current methodologies do not distinguish between fats serving the energy needs of the parent, akin to mammalian adiposity, from those that are distributed to the progeny, making it difficult to accurately interpret the physiological implications of fat content changes induced by external perturbations. Using spectroscopic coherent Raman imaging, we determine the protein content, chemical profiles and dynamics of lipid particles in live animals. We find fat particles in the adult intestine to be diverse, with most destined for the developing progeny. In contrast, the skin-like epidermis contains fats that are the least heterogeneous, the least dynamic and have high triglyceride content. These attributes are most consistent with stored somatic energy reservoirs. These results challenge the prevailing practice of assessing C. elegans adiposity by measurements that are dominated by the intestinal fat content. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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