Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

Autor: Sierra, RG, Gati, C, Laksmono, H, Dao, EH, Gul, S, Fuller, F, Kern, J, Chatterjee, R, Ibrahim, M, Brewster, AS, Young, ID, Michels-Clark, T, Aquila, A, Liang, M, Hunter, MS, Koglin, JE, Boutet, S, Junco, EA, Hayes, B, Bogan, MJ, Hampton, CY, Puglisi, EV, Sauter, NK, Stan, CA, Zouni, A, Yano, J, Yachandra, VK, Soltis, SM, Puglisi, JD, DeMirci, H
Jazyk: angličtina
Rok vydání: 2015
Zdroj: Sierra, RG; Gati, C; Laksmono, H; Dao, EH; Gul, S; Fuller, F; et al.(2015). Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. Nature Methods, 13(1), 59-62. doi: 10.1038/nmeth.3667. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/9kp8r8ks
Popis: We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (
Databáze: OpenAIRE